Table 1.

Alignment on amino acids appearing in eight subsites of memapsin substrates

Peptide	Subsites
	P4	P3	P2	P1	P1′	P2′	P3′	P4′
APP β	E	V	K	M	D	A	E	F
Swedish APP β	E	V	N	L	D	A	E	F
PS-1	L	V	N	M	A	E	G	D
M2-Pro	R	G	S	M	A	G	V	L
	G	T	Q	H	G	I	R	L
M2	S	S	N	F	A	V	G	A
	G	L	A	Y	A	E	I	A
Insulin B-chain	H	L	C*	G	S	H	L	V
	C*	G	E	R	G	F	F	Y
	E	A	L	Y	L	V	C*	G
Nch†	G	V	L	L	S	R	K
		V	G	S	G	V	L	L
				V	G	S	G	V

^*C is cysteic acid. 

^† Nch sequence VGSGVLLSRK.