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. 2009 Mar 2;4(3):e4660. doi: 10.1371/journal.pone.0004660

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Lim et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Positive control (PC) consists of lung nuclear proteins from IL4Ra+/− embryos directly injected with IL-13. Negative controls consist of lung nuclear protein from IL4Ra−/− females injected with IL-13 (NC1) and nuclear protein from IL4Ra+/− embryos injected with PBS (NC2). The experimental group consists of nuclear proteins from IL4Ra+/− embryos whose mothers were injected with IL-13. Bands for P-STAT-6 are only detectable in PC lane. HDAC1 was used as a loading control. Insufficient protein was loaded in NC1, but otherwise protein loading was similar.