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. 2009 Mar 2;4(3):e4660. doi: 10.1371/journal.pone.0004660

Figure 2. STAT-6 phosphorylation following injection with IL-13.

Figure 2

Positive control (PC) consists of lung nuclear proteins from IL4Ra+/− embryos directly injected with IL-13. Negative controls consist of lung nuclear protein from IL4Ra−/− females injected with IL-13 (NC1) and nuclear protein from IL4Ra+/− embryos injected with PBS (NC2). The experimental group consists of nuclear proteins from IL4Ra+/− embryos whose mothers were injected with IL-13. Bands for P-STAT-6 are only detectable in PC lane. HDAC1 was used as a loading control. Insufficient protein was loaded in NC1, but otherwise protein loading was similar.