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. 2005 Jan-Mar;2(1):17–21. doi: 10.4161/org.2.1.1726

Figure 2.

Figure 2

Effect of endogenous VEGF and FGF2 sequestering by specific antibodies on UB growth in metanephroi cultured under HC. UBs were stained using by FITC-conjugated DBA. (A) Metanephros freshly dissected from 12-day embryo. Metanephros cultured for two days in control media (B, control) or control media plus 1 µg/ml αmVEGF (C), 25 µg/ml αhFGF2 (D), or a combination of two (E). (F) Quantitative analysis of the inhibition of UB branching. (G) Metanephros protein content. Data shown in (A–E) are representative. Data shown in (F and G) are means ± SEM of four independent experiments. **p < 0.01 versus control.