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. 2005 Jan-Mar;2(1):17–21. doi: 10.4161/org.2.1.1726

Figure 3.

Figure 3

Effect of added exogenous VEGF and FGF2 on metanephroi cultured under RA. UBs were stained using FITC-conjugated DBA lectin. (A) Metanephroi freshly dissected from 12-day embryo. Metanephroi cultured for two days under RA in control media (B, control) or in the presence of 20 ng rhVEGF (C), 20 ng/ml rhFGF2 (D), or a combination of the two (E). (F) Quantitative analysis of UB branching. (G) Protein content. Data shown in A–E are representative. Data shown in (F and G) are means ± SEM of four independent experiments. **p < 0.01 versus control.