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Genotyping the ngn3-EGFP 1.37 line. Genotyping PCR was carried out on tail tip DNA to check for the presence of both EGFP (483 bp) and neomycin (neo) (410 bp) constructs in the offspring of the founder mice. +/+, positive for EGFP and Neomycin transgenes; −/−, negative for EGFP and Neomycin transgenes.
