Reverse transcriptase PCR (RT/PCR) analysis of the purified EGFP-positive cells from an E15.5 pancreas. Ethidium bromide stained agarose gel of RT/PCR products from total RNA isolated from presorted FACS purified EGFP-positive cells, and the total population of enzyme dispersed cells from EGFP-negative embryos from the same litter. The control panel shows the reaction in the absence of reverse transcriptase. (A) b actin, EGFP and ngn3; (B) The results for insulin 1 (INS1), insulin 2 (INS2), glucagon (GLU), somatostatin (SOM) and pancreatic polypeptide (PP).