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Reactive oxygen species (ROS) induce MUC5B protein. Normal human bronchial epithelial (NHBE) cells exposed to PBS or X/XO in the presence or the absence of catalase (Cat) were labeled using antibodies against MUC5B (green) and MUC5AC (red) and analyzed using fluorescence microscopy (magnification: ×20). Red arrow indicates a cell immunolabeled only with MUC5AC; green arrow shows a cell only positive for MUC5B; and orange arrow denotes a cell where MUC5AC and MUC5B are co-localized.
