Figure 1.

Clara cell depletion in naphthalene and HSVtk models. (A) Wild-type or (B) CCSP-HSVtk transgenic mice were exposed to naphthalene (275 mg/kg) or ganciclovir, respectively, and expression of CCSP, Cyp2f2, and Sftpc was assayed by quantitative real-time PCR in total lung RNA (n = 4). All RT-PCR data are shown normalized to % mean mRNA abundance within unexposed control mice, with error bars indicating SEM. (A) Naphthalene exposure caused a 90% reduction in both CCSP and Cyp2f2 mRNA abundance by Day 2 (P < 0.05 for both CCSP and Cyp2f2, control versus later time points by one-way ANOVA). No statistically significant change was seen in Sftpc mRNA abundance. (B) Ganciclovir-mediated Clara cell ablation decreased CCSP and Cyp2f2 mRNA abundance to less than 10% of untreated control by Recovery Day 9 (P < 0.05 for both CCSP and Cyp2f2, control versus later time points by one-way ANOVA). No statistically significant decrease in Sftpc mRNA abundance was seen with ganciclovir exposure.