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. Author manuscript; available in PMC: 2009 Feb 20.
Published in final edited form as: Ann N Y Acad Sci. 2008 Dec;1147:275–282. doi: 10.1196/annals.1427.028

Figure 2.

Figure 2

Bioenergetic effects of mild mitochondrial uncoupling and caloric restriction. (A) [14C]-2-deoxyglucose uptake was significantly increased in cortical neurons treated with the mitochondrial uncoupler 2, 4-DNP (20 μmol/L). Values are the mean and SD of measurements made in 4 cultures. (B) Cultured cortical neurons were loaded with TMRE and time-lapse confocal images of TMRE fluorescence (gray) and NAD(P)H autofluorescence (black) were acquired sequentially at 10-s intervals. DNP induced a transient increase of NAD(P)H autofluorescence followed by a prolonged decrease from enhanced NAD(P)H oxidation. (C, D)In-creased glucose transporter 1 (GLUT1) mRNA expression (in situ hybridization with 35S-labeled riboprobes) was detected in caloric restricted (CR) mouse brains (6-month-old, 3 months under alternate-day fasting) compared to noncaloric restricted control (AL, ad libitum); n = 5 animals in each group.