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Oxidative aggregation interferes with fibril formation. (A) Molecular weight marker. (B) The wild-type protein was reacted with cytochrome c and H2O2 before (lanes 1 and 2) or after (lanes 3 and 4) fibril formation. Fibrils were isolated by centrifugation. Fibrils (lanes 2 and 4) and the supernatant (lanes 1 and 3) were boiled with SDS, separated on 10–20% gradient polyacrylamide gels, and visualized with Coomassie Blue.
