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. 1983 Oct;42(1):293–300. doi: 10.1128/iai.42.1.293-300.1983

Response of mice to rotaviruses of bovine or primate origin assessed by radioimmunoassay, radioimmunoprecipitation, and plaque reduction neutralization.

P A Offit, H F Clark, S A Plotkin
PMCID: PMC264557  PMID: 6311747

Abstract

Sera from (i) gnotobiotic BALB/c, CD-1, and CFW mice and (ii) conventional BALB/c mice were evaluated by radioimmunoassay, radioimmunoprecipitation, and plaque reduction neutralization, using the Wa, SA-11, and WC-3 (bovine) strains of rotavirus as the detecting antigens. The gnotobiotic mice had no antirotavirus antibody detectable by radioimmunoprecipitation and no neutralizing antibody at a dilution of 1:50 by plaque reduction neutralization. All sera from the conventional mice had rotavirus-specific antibodies detected by radioimmunoassay and by radioimmunoprecipitation at serum dilutions of 1:50 and 1:10,000, respectively. The antibodies were directed against viral proteins p116, p94, p88, and p84 of all three viruses, but had no neutralizing activity against heterologous rotaviruses at a dilution of 1:50. Conventional seropositive mice were parenterally immunized with the Wa, SA-11, or WC-3 strain of rotavirus. An approximate 100-fold increase in rotavirus-specific antibodies was detected by radioimmunoassay, and greater than 20-fold selective neutralization of the immunizing strain of virus was observed. Sera from the mice immunized with Wa virus had antibodies directed against inner and outer capsid proteins of all three rotaviruses. The mouse can be a useful model for studying the immune response to heterologous rotavirus infection; preexisting antibodies presumably directed towards murine rotavirus do not prevent the development of a type-specific immune response to a nonmurine rotavirus.

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Selected References

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  1. Bastardo J. W., McKimm-Breschkin J. L., Sonza S., Mercer L. D., Holmes I. H. Preparation and characterization of antisera to electrophoretically purified SA11 virus polypeptides. Infect Immun. 1981 Dec;34(3):641–647. doi: 10.1128/iai.34.3.641-647.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bridger J. C. Location of type-specific antigens in calf rotaviruses. J Clin Microbiol. 1978 Dec;8(6):625–628. doi: 10.1128/jcm.8.6.625-628.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bryden A. S., Davies H. A., Hadley R. E., Flewett T. H. Rotavirus enteritis in the West Midlands during 1974. Lancet. 1975 Aug 9;2(7928):241–243. doi: 10.1016/S0140-6736(75)90959-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davidson G. P., Bishop R. F., Townley R. R., Holmes I. H. Importance of a new virus in acute sporadic enteritis in children. Lancet. 1975 Feb 1;1(7901):242–246. doi: 10.1016/s0140-6736(75)91140-x. [DOI] [PubMed] [Google Scholar]
  5. Elias M. M. Distribution and titres of rotavirus antibodies in different age groups. J Hyg (Lond) 1977 Dec;79(3):365–372. doi: 10.1017/s0022172400053201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ericson B. L., Graham D. Y., Mason B. B., Estes M. K. Identification, synthesis, and modifications of simian rotavirus SA11 polypeptides in infected cells. J Virol. 1982 Jun;42(3):825–839. doi: 10.1128/jvi.42.3.825-839.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Estes M. K., Graham D. Y. Identification of rotaviruses of different origins by the plaque-reduction test. Am J Vet Res. 1980 Jan;41(1):151–152. [PubMed] [Google Scholar]
  8. Gaul S. K., Simpson T. F., Woode G. N., Fulton R. W. Antigenic relationships among some animal rotaviruses: virus neutralization in vitro and cross-protection in piglets. J Clin Microbiol. 1982 Sep;16(3):495–503. doi: 10.1128/jcm.16.3.495-503.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Greenberg H. B., Kalica A. R., Wyatt R. G., Jones R. W., Kapikian A. Z., Chanock R. M. Rescue of noncultivatable human rotavirus by gene reassortment during mixed infection with ts mutants of a cultivatable bovine rotavirus. Proc Natl Acad Sci U S A. 1981 Jan;78(1):420–424. doi: 10.1073/pnas.78.1.420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hall G. A., Bridger J. C., Chandler R. L., Woode G. N. Gnotobiotic piglets experimentally infected with neonatal calf diarrhoea reovirus-like agent (Rotavirus). Vet Pathol. 1976;13(3):197–210. doi: 10.1177/030098587601300304. [DOI] [PubMed] [Google Scholar]
  11. Hoshino Y., Wyatt R. G., Scott F. W., Appel M. J. Isolation and characterization of a canine rotavirus. Arch Virol. 1982;72(1-2):113–125. doi: 10.1007/BF01314456. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kalica A. R., Greenberg H. B., Wyatt R. G., Flores J., Sereno M. M., Kapikian A. Z., Chanock R. M. Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology. 1981 Jul 30;112(2):385–390. doi: 10.1016/0042-6822(81)90285-3. [DOI] [PubMed] [Google Scholar]
  13. Kapikian A. Z., Kim H. W., Wyatt R. G., Cline W. L., Arrobio J. O., Brandt C. D., Rodriguez W. J., Sack D. A., Chanock R. M., Parrott R. H. Human reovirus-like agent as the major pathogen associated with "winter" gastroenteritis in hospitalized infants and young children. N Engl J Med. 1976 Apr 29;294(18):965–972. doi: 10.1056/NEJM197604292941801. [DOI] [PubMed] [Google Scholar]
  14. Kapikian A. Z., Wyatt R. G., Greenberg H. B., Kalica A. R., Kim H. W., Brandt C. D., Rodriguez W. J., Parrott R. H., Chanock R. M. Approaches to immunization of infants and young children against gastroenteritis due to rotaviruses. Rev Infect Dis. 1980 May-Jun;2(3):459–469. doi: 10.1093/clinids/2.3.459. [DOI] [PubMed] [Google Scholar]
  15. Kapikian A. Z., Wyatt R. G., Levine M. M., Yolken R. H., VanKirk D. H., Dolin R., Greenberg H. B., Chanock R. M. Oral administration of human rotavirus to volunteers: induction of illness and correlates of resistance. J Infect Dis. 1983 Jan;147(1):95–106. doi: 10.1093/infdis/147.1.95. [DOI] [PubMed] [Google Scholar]
  16. Killen H. M., Dimmock N. J. Identification of a neutralization-specific antigen of a calf rotavirus. J Gen Virol. 1982 Oct;62(Pt 2):297–311. doi: 10.1099/0022-1317-62-2-297. [DOI] [PubMed] [Google Scholar]
  17. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  18. Lecce J. G., King M. W., Dorsey W. E. Rearing regimen producing piglet diarrhea (rotavirus) and its relevance to acute infantile diarrhea. Science. 1978 Feb 17;199(4330):776–778. doi: 10.1126/science.203032. [DOI] [PubMed] [Google Scholar]
  19. MACPHERSON I., STOKER M. Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence. Virology. 1962 Feb;16:147–151. doi: 10.1016/0042-6822(62)90290-8. [DOI] [PubMed] [Google Scholar]
  20. Mason B. B., Graham D. Y., Estes M. K. Biochemical mapping of the simian rotavirus SA11 genome. J Virol. 1983 May;46(2):413–423. doi: 10.1128/jvi.46.2.413-423.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mason B. B., Graham D. Y., Estes M. K. In vitro transcription and translation of simian rotavirus SA11 gene products. J Virol. 1980 Mar;33(3):1111–1121. doi: 10.1128/jvi.33.3.1111-1121.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Mathan M., Almeida J. D., Cole J. An antigenic subunit present in rotavirus infected faeces. J Gen Virol. 1977 Feb;34(2):325–329. doi: 10.1099/0022-1317-34-2-325. [DOI] [PubMed] [Google Scholar]
  23. Matsuno S., Inouye S., Kono R. Antigenic relationship between human and bovine rotaviruses as determined by neutralization, immune adherence hemagglutination, and complement fixation tests. Infect Immun. 1977 Sep;17(3):661–662. doi: 10.1128/iai.17.3.661-662.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Matsuno S., Inouye S., Kono R. Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera. J Clin Microbiol. 1977 Jan;5(1):1–4. doi: 10.1128/jcm.5.1.1-4.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Matsuno S., Inouye S. Purification of an outer capsid glycoprotein of neonatal calf diarrhea virus and preparation of its antisera. Infect Immun. 1983 Jan;39(1):155–158. doi: 10.1128/iai.39.1.155-158.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. McNulty M. S., Allan G. M., Thompson D. J., O'Boyle J. D. Antibody to rotavirus in dogs and cats. Vet Rec. 1978 Jun 17;102(24):534–535. doi: 10.1136/vr.102.24.534. [DOI] [PubMed] [Google Scholar]
  27. Merril C. R., Goldman D., Sedman S. A., Ebert M. H. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science. 1981 Mar 27;211(4489):1437–1438. doi: 10.1126/science.6162199. [DOI] [PubMed] [Google Scholar]
  28. Middleton P. J., Szymanski M. T., Petric M. Viruses associated with acute gastroenteritis in young children. Am J Dis Child. 1977 Jul;131(7):733–737. doi: 10.1001/archpedi.1977.02120200015004. [DOI] [PubMed] [Google Scholar]
  29. Petric M., Middleton P. J., Grant C., Tam J. S., Hewitt C. M. Lapine rotavirus: preliminary studies on epizoology and transmission. Can J Comp Med. 1978 Jan;42(1):143–147. [PMC free article] [PubMed] [Google Scholar]
  30. Sato K., Inaba Y., Miura Y., Tokuhisa S., Matumoto M. Antigenic relationships between rotaviruses from different species as studied by neutralization and immunofluorescence. Arch Virol. 1982;73(1):45–50. doi: 10.1007/BF01341726. [DOI] [PubMed] [Google Scholar]
  31. Sato K., Inaba Y., Miura Y., Tokuhisa S., Matumoto M. Isolation of lapine rotavirus in cell cultures. Brief report. Arch Virol. 1982;71(3):267–271. doi: 10.1007/BF01314878. [DOI] [PubMed] [Google Scholar]
  32. Sheridan J. F., Eydelloth R. S., Vonderfecht S. L., Aurelian L. Virus-specific immunity in neonatal and adult mouse rotavirus infection. Infect Immun. 1983 Feb;39(2):917–927. doi: 10.1128/iai.39.2.917-927.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Smith R. E., Zweerink H. J., Joklik W. K. Polypeptide components of virions, top component and cores of reovirus type 3. Virology. 1969 Dec;39(4):791–810. doi: 10.1016/0042-6822(69)90017-8. [DOI] [PubMed] [Google Scholar]
  34. Snodgrass D. R., Herring J. A., Linklater K. A., Dyson D. A. A survey of rotaviruses in sheep in Scotland. Vet Rec. 1977 Apr 16;100(16):341–341. doi: 10.1136/vr.100.16.341-a. [DOI] [PubMed] [Google Scholar]
  35. Stuker G., Schmidt N. J., Forghani B., Holmberg C. A., Henrickson R. V., Anderson J. H. Rotavirus antibody assays on monkey sera: a comparison of enzyme immunoassay with neutralization and complement-fixation tests. Am J Vet Res. 1979 Nov;40(11):1620–1623. [PubMed] [Google Scholar]
  36. Thouless M. E., Beards G. M., Flewett T. H. Serotyping and subgrouping of rotavirus strains by the ELISA test. Arch Virol. 1982;73(3-4):219–230. doi: 10.1007/BF01318076. [DOI] [PubMed] [Google Scholar]
  37. Thouless M. E., Bryden A. S., Flewett T. H., Woode G. N., Bridger J. C., Snodgrass D. R., Herring J. A. Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization. Arch Virol. 1977;53(4):287–294. doi: 10.1007/BF01315627. [DOI] [PubMed] [Google Scholar]
  38. Urasawa S., Urasawa T., Taniguchi K. Three human rotavirus serotypes demonstrated by plaque neutralization of isolated strains. Infect Immun. 1982 Nov;38(2):781–784. doi: 10.1128/iai.38.2.781-784.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Woode G. N., Bridger J. C., Jones J. M., Flewett T. H., Davies H. A., Davis H. A., White G. B. Morphological and antigenic relationships between viruses (rotaviruses) from acute gastroenteritis of children, calves, piglets, mice, and foals. Infect Immun. 1976 Sep;14(3):804–810. doi: 10.1128/iai.14.3.804-810.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Woode G. N. Epizootiology of bovine rotavirus infection. Vet Rec. 1978 Jul 15;103(3):44–46. doi: 10.1136/vr.103.3.44. [DOI] [PubMed] [Google Scholar]
  41. Wyatt R. G., Greenberg H. B., James W. D., Pittman A. L., Kalica A. R., Flores J., Chanock R. M., Kapikian A. Z. Definition of human rotavirus serotypes by plaque reduction assay. Infect Immun. 1982 Jul;37(1):110–115. doi: 10.1128/iai.37.1.110-115.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Yolken R. H., Barbour B., Wyatt R. G., Kalica A. R., Kapikian A. Z., Chanock R. M. Enzyme-linked immunosorbent assay for identification of rotaviruses from different animal species. Science. 1978 Jul 21;201(4352):259–262. doi: 10.1126/science.208150. [DOI] [PubMed] [Google Scholar]

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