Figure 3.
Western blot analysis of non-PK treated brain homogenates following capture ELISA assay. The capture ELISA assay was carried out on normal sheep (NS), scrapie-infected sheep (SS), normal deer (ND), CWD-infected deer (CWD), normal hamster (NH) and 263K-infected hamsters (263K) under the same conditions as described for Figure 2 using a non-biotinylated detection reagent. The material was eluted from the microtiter plate as described in Materials and Methods and Western blotted. Immunostaining was carried out using Mab 8E9.