. Author manuscript; available in PMC: 2009 Dec 15.

Published in final edited form as: J Neuroimmunol. 2008 Oct 31;205(1-2):94–100. doi: 10.1016/j.jneuroim.2008.09.013

Table 1.

Comparision of Denaturation Treatments on PrP^Sc Immunoreactivity¹.

Treatment	Signal Intensity²
	5D6	11F12	8E9
Untreated	0.214 ± 0.017	0.339 ± 0.009	0.303 ± 0.110
SDS³	1.229 ± 0.019	1.599 ± 0.182	1.324 ± 0.251
Heat⁴	1.228 ± 0.018	1.085 ± 0.124	1.427 ± 0.331
SDS + Heat	2.232 ± 0.008	2.548 ± 0.112	2.821 ± 0.252

PrP^Sc was purified from the brains of clinical hamsters infected with the hamster adapted 263K scrapie strain and PK treated as described in Materials and Methods.

Signal intensity was measured by indirect ELISA assay at OD₄₀₅.

SDS was added to a final concentration of 1%.

⁴

Samples were heated at 100^o C for 5 min.