Table 1.

Measurements of the affinity of MgADP for S1 at 0°C, in liter/mol × 10⁵, for native S1 (N), S1 modified with dibromobimane (D), and monobromobimane (MBB)

Measurement	Mean ± SD
N (n = 11): 1.65, 0.41, 0.21, 1.36, 0.69, 0.20, 0.61, 0.64, 0.19, 0.93, 0.26	0.64  ± 0.47
D (n = 7): 0.51, 0.16, 0.09, 0.25, 0.15, 0.18, 0.16	0.22  ± 0.13
M (n = 4): 0.11, 0.26, 0.28, 0.11	0.19  ± 0.07

The solvent is 50 mM Pipes/100 mM KCl/5 mM MgCl₂/0.1 mM EDTA/0.1 mM EGTA/2 mM NaN₃, in 30% D₂O, pH 7.09 (at 0°C before adding D₂O).