Figure 6. The binding of SATB1 at MARs of β-globin gene cluster in metaphase.

The occupancy of SATB1(A), acetylated-H3 (H3Ac) (B) and RNP(C) at β-like globin gene clusters in asynchronous (As) and synchronous (s) K562 cells. The occupancies of SATB1 at target sites of β-globin clusters and c-myc gene in (As) and (s) cell populations were compared after normalizing the DNA amount differences in (As) and (s) populations through DNA input (In-As and In-s). Necdin was chosen as negative control of ChIP assay. The ε-pro indicates that the ChIP primer position is at the ε-globin promoter region. HS2core indicates that the ChIP primer position is at the core region of HS2. Error bars represent the standard deviations of at least 3 replicates.