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. 2009 Feb 26;4(2):e4613. doi: 10.1371/journal.pone.0004613

Figure 3. MAGI-1L regulates GLR-1 localization in response to prior experience.

Figure 3

(A) Timeline for the stimulation protocol. Gray boxes indicate periods of vibration-free isolation, whereas the white box indicates a period of mechanostimulation. Each arrow indicates a set of 15 mechanical shocks delivered one minute apart. (B) GLR-1::GFP puncta in wild type and magi-1(tm446) mutants, both undisturbed animals (young adult) and animals mechanically stimulated the previous day. Ventral aspect, anterior is to the left. Bar, 5 µm. The mean (C,F) number per 100 microns, (D) size, and (E) intensity of GLR-1::GFP fluorescent puncta in the anterior cord was quantified (see Methods). (G,H) The mean spontaneous reversal frequency of animals is indicated. (C–H) Animals were either unstimulated (solid bars) or previously exposed to mechanical stimulation (stippled bars). Error bars indicate s.e.m. (C,D,G) ***p<0.0001, *p<0.01, Factorial ANOVA. (F,H) ***p<0.001, **p<0.01, One-way ANOVA followed by Dunnett's posthoc comparison to wild type, n = 15–25 for each genotype/condition. Data is pooled from three independent transgenic lines.