FIGURE 3.

The RTD1a nonamer sequence is sufficient for interaction with SDF2L1. A, schematic representation of the DBD-fusion constructs of proRTD1a and its mutants, described in Table 1. B, spot tests were performed using three colonies of AH109 cells transformed with plasmids listed. C, immunoprecipitation was performed by incubating extracts of AH109 cells containing DBD-vector (lane 1), DBD-proRTD1a (lane 2), and DBD-proseg (lane 3) with anti-DBD antibodies and protein A-Sepharose. The immunoblot was probed using anti-Myc antibodies. The upper band present in all three lanes is antibody heavy chain.