The RTD1a nonamer sequence is sufficient for interaction with
SDF2L1. A, schematic representation of the DBD-fusion constructs
of proRTD1a and its mutants, described in
Table 1. B, spot tests
were performed using three colonies of AH109 cells transformed with plasmids
listed. C, immunoprecipitation was performed by incubating extracts
of AH109 cells containing DBD-vector (lane 1), DBD-proRTD1a (lane
2), and DBD-proseg (lane 3) with anti-DBD antibodies and protein
A-Sepharose. The immunoblot was probed using anti-Myc antibodies. The
upper band present in all three lanes is antibody heavy chain.