TABLE 1.
Plasmids and yeast strains used in this study
| Plasmid (description) | Comments |
| PEC2036 (AD-vector) | pGADT7-Rec from Clontech TRP1 Ampr |
| PEC2037 (DBD-vector) | pGBKT7 from Clontech LEU2 Kanr |
| PEC2038 (DBD-proRTD1a) | proRTD1a cloned in the NcoI/BamHI sites of pGBKT7 |
| PEC2069 (AD-SDF2L1) | SDF2L1/pGADT7-Rec; plasmid expressing interactor of proRTD1a, isolated from yeast |
| PEC2073 (DBD-proseg) | The prosegment of RTD1a cloned in the EcoRI/BamHI sites of pGBKT7 |
| PEC2082 (DBD-proHNP3) | PEC2080 (proHNP3/pCR2.1) was digested with Acc65I, filled-in with Klenow and then digested with EcoRI. The fragment containing proHNP3 was then ligated into EcoRI/SmaI-digested pGBKT7 |
| PEC2084 (AD-ΔN28SDF2L1) | ΔN28SDF2L1 cloned into EcoRI and XhoI sites of pGADT7-Rec |
| PEC2105 (AD-ΔN28SDF2L1ΔMIR1) | SDF2L1ΔMIR1 (N-terminal deletion, ΔN87) cloned into EcoRI and XhoI sites of pGADT7-Rec |
| PEC2106 (AD-SDF2L1ΔMIR3) | SDF2L1ΔMIR3 (C-terminal deletion, ΔC151) cloned into EcoRI and XhoI sites of pGADT7-Rec |
| PEC2113 (DBD-proHBD1) | proHBD1 cloned as EcoRI/BamHI fragment into pGBKT7 |
| PEC2114 (DBD-proHD5) | proHD5 cloned as EcoRI/BamHI fragment into pGBKT7 |
| PEC2122 (DBD-ΔN28SDF2L1ΔMIR2) | ΔN28SDF2L1ΔMIR2 (internal deletion of residues 95-149) cloned into the EcoR1/XhoI sites of pGADT7-Rec |
| PEC2123 (DBD-proHNP3Δ35-49) | proHNP3Δ35-49 fragment cloned into EcoRI/BamHI sites of pGBKT7 |
| PEC2124 (DBD-proRTD1aΔRLL) | proRTD1aΔRLL cloned into NcoI and BamHI sites of pGBKT7 |
| PEC2125 (DBD-RTD1a-9mer) | RTD1a was cloned into the EcoRI and XhoI sites of pGBKT7 |
| PEC2126 (DBD-HNP3) | HNP3 fragment was cloned into EcoRI and XhoI sites of pGBKT7 |
| PEC2127 (AD-MIR3) | MIR3 fragment (residues 151-221 of SDF2L1) was cloned into EcoRI and XhoI sites of pGADT7-Rec |
| PEC2003 (GST) | NcoI site in PEC615 (UAF30/pCBGST1) was filled in with Klenow and re-ligated to render the UAF30 sequence out of frame, for expression of GST TRP1 Ampr |
| PEC2007 (GST-proRTD1a) | proRTD1a cloned in the NcoI/Acc65I sites of pCBGST1 for expression of GST-proRTD1a |
| PEC2075 (GST-ΔN28SDF2L1) | ΔN28SDF2L1 cloned into the NcoI/Acc65I sites of pCBGST1 for expression of GST-ΔN28SDF2L1 |
| PEC2121 (GST-proHNP3) | proHNP3 cloned into the NcoI/Acc65I sites of pCBGST1 for expression of GST-proHNP3 |
| Yeast strains | Phenotype |
| AH109 | MATa trp1-901 leu2-3, 112 ura3-52 his3-Δ200 gal4Δ gal80Δ LYS2::GAL1UAS-GAL1TATA-HIS3 GAL2UAS-GAL2TATA-ADE2 URA3::MEL1UAS-MEL1TATA-lacZ MEL1 |
| JD47-13C | MATa his3-Δ200 trp1-Δ63 lys2-801 ura3-52 leu2-3, 112 |