PEC2036 (AD-vector)
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pGADT7-Rec from Clontech TRP1 Ampr
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PEC2037 (DBD-vector)
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pGBKT7 from Clontech LEU2 Kanr
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PEC2038 (DBD-proRTD1a)
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proRTD1a cloned in the NcoI/BamHI sites of pGBKT7
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PEC2069 (AD-SDF2L1)
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SDF2L1/pGADT7-Rec; plasmid expressing interactor of proRTD1a, isolated from
yeast
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PEC2073 (DBD-proseg)
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The prosegment of RTD1a cloned in the EcoRI/BamHI sites of
pGBKT7
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PEC2082 (DBD-proHNP3)
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PEC2080 (proHNP3/pCR2.1) was digested with Acc65I, filled-in with
Klenow and then digested with EcoRI. The fragment containing proHNP3
was then ligated into EcoRI/SmaI-digested pGBKT7
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PEC2084 (AD-ΔN28SDF2L1)
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ΔN28SDF2L1 cloned into EcoRI and XhoI sites
of pGADT7-Rec
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PEC2105 (AD-ΔN28SDF2L1ΔMIR1)
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SDF2L1ΔMIR1 (N-terminal deletion, ΔN87) cloned into
EcoRI and XhoI sites of pGADT7-Rec
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PEC2106 (AD-SDF2L1ΔMIR3)
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SDF2L1ΔMIR3 (C-terminal deletion, ΔC151) cloned into
EcoRI and XhoI sites of pGADT7-Rec
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PEC2113 (DBD-proHBD1)
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proHBD1 cloned as EcoRI/BamHI fragment into pGBKT7
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PEC2114 (DBD-proHD5)
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proHD5 cloned as EcoRI/BamHI fragment into pGBKT7
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PEC2122 (DBD-ΔN28SDF2L1ΔMIR2)
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ΔN28SDF2L1ΔMIR2 (internal deletion of residues 95-149)
cloned into the EcoR1/XhoI sites of pGADT7-Rec
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PEC2123 (DBD-proHNP3Δ35-49)
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proHNP3Δ35-49 fragment cloned into EcoRI/BamHI sites
of pGBKT7
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PEC2124 (DBD-proRTD1aΔRLL)
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proRTD1aΔRLL cloned into NcoI and BamHI sites of
pGBKT7
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PEC2125 (DBD-RTD1a-9mer)
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RTD1a was cloned into the EcoRI and XhoI sites of pGBKT7
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PEC2126 (DBD-HNP3)
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HNP3 fragment was cloned into EcoRI and XhoI sites of pGBKT7
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PEC2127 (AD-MIR3)
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MIR3 fragment (residues 151-221 of SDF2L1) was cloned into EcoRI and
XhoI sites of pGADT7-Rec
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PEC2003 (GST)
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NcoI site in PEC615 (UAF30/pCBGST1) was filled in with
Klenow and re-ligated to render the UAF30 sequence out of frame, for
expression of GST TRP1 Ampr
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PEC2007 (GST-proRTD1a)
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proRTD1a cloned in the NcoI/Acc65I sites of pCBGST1 for
expression of GST-proRTD1a
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PEC2075 (GST-ΔN28SDF2L1)
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ΔN28SDF2L1 cloned into the NcoI/Acc65I
sites of pCBGST1 for expression of GST-ΔN28SDF2L1
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PEC2121 (GST-proHNP3)
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proHNP3 cloned into the NcoI/Acc65I sites of pCBGST1 for
expression of GST-proHNP3
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