FIGURE 5.

Hepatocellular apoptosis is increased in SCD1^–/– mice on the MCD diet. To assess if the changes in lipid partitioning were associated with increase apoptotic cell death as we observed in cultured hepatocytes, hepatocellular apoptosis was quantified. A, representative microphotograph of TUNEL staining of liver section from SCD1^–/– and SCD1^+/+ mice on either the MCD or control diet. B, quantitation of TUNEL staining in liver sections from the four groups of mice by counting the number of TUNEL-positive cells in 10 random microscopic fields. Results are expressed as mean ± S.D.