TABLE 1.

Tryptic peptides of AAF-132 and AAF-44 and an N-terminal peptide of AAF-44

AAF was purified from L1210 cells as described previously (11). After SDS-PAGE, the bands corresponding to AAF-132 and AAF-44 were excised, and tryptic fragments were analyzed by tandem mass spectrometry or N-terminal Edman degradation (AAF-44 peptide 1) as described under “Experimental Procedures.”

	Identified as amino acid residues
AAF-132 peptide fragments
1 GGRPGDGVLPR	98-108
2 QLILLGTLVDLLGDSEQE	110-127
3 AWATFDPK	561-568
4 LVAPGPSTPVFETEGSSGT	814-832
5 XHFSQLEK	962-968
6 ALGLSPSEWSSILEHAR	1090-1106
7 VALQFTGLGGQTXSASK	1111-1127
8 THEPLTLLLR	1128-1137
9 PTDISPR	1159-1165
AAF-44 peptide fragments
1 PQPXLXME	2-9
2 IGIGDIIR	136-143
3 XIXANIYYK	157-164
4 XDDPVWNMQIAR	166-176
5 LQEEEALNNKDNLDLAGLTSLLSEK	197-221
6 VLELLEDQSDIVSTADHYY	357-375