FIGURE 5.

Rab2 and aPKCι co-distribute with dynein. HeLa cells were transfected with either Rab2 cDNA or PKCι cDNA, as described under “Experimental Procedures.” aPKCι overexpressing cells were permeabilized, fixed, and then co-stained with anti-dynein (intermediate chain) and fluorescein isothiocyanate-anti-Xpress (A) or fluorescein isothiocyanate anti-Xpress and anti-Tyr-tubulin (C). B, Rab2 overexpressing cells processed as above were co-labeled with anti-dynein (intermediate chain) and anti-Rab2. Boxed areas (yellow indicates co-localization) were analyzed using the Manders overlap co-localization, as described under “Experimental Procedures” and reported under “Results.” Both Rab2 and aPKCι co-distribute with dynein in perinuclear pleomorphic entities.