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Inactivation of hCPSI WT, C1327A, and C1337A by thiol selective reagents
The reaction mixtures contained 2.5 μm enzyme, 5.5 μm PAO, or 5.2 μm PySSPy and where indicated 2 mm AGA or 7 mm ATP + 12 mm MgCl2. Aliquots were removed at the indicated times of incubation at 30 °C and assayed for CP synthesis activity.
