FIGURE 1.
MMTV-Cre-mediated conditional KO of FIP200 does not affect lymphomagenesis induced by p53 inactivation. A, lysates were prepared from mammary glands of the first lactation day of FIP200 CKO and FIP200F/F mice and analyzed by Western blotting using anti-FIP200 (top) or anti-actin (bottom). B, Kaplan-Meier analysis of tumor development in mice with the indicated genotypes. n represents the number of animals analyzed. C, representative micrographs of lymphoma in thymus (a), lung (b), spleen (c), and kidney (d) in dCKO mice. Scale bar = 50 μm. D, lysates were prepared from thymus of 4-week-old mice and analyzed by Western blotting using anti-FIP200 (upper), anti-p53 (middle), or anti-vinculin (lower) antibodies. The asterisk marks a background band. E, lysates prepared from thymic lymphoma samples in p53 CKO (lanes 2-5) and dCKO (lanes 6-9) mice or thymus of wild type mice (lane 1) were analyzed by Western blotting using anti-FIP200 (upper), anti-p53 (middle), or anti-vinculin (lower) antibodies.