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. Author manuscript; available in PMC: 2009 Feb 20.
Published in final edited form as: Brain Res. 2006 Nov 27;1128(1):79–85. doi: 10.1016/j.brainres.2006.10.047

Figure 2. Effect of 3Me-H TRH on glutamate toxicity as measured by cell counts (a) and LDH release (b) in cultured fetal hippocampal neurons.

Figure 2

Cultured fetal (E17) hippocampal neurons were either untreated or exposed to varying concentrations of 3Me-H TRH and/or 500 μM Glu for 16 hrs After 16 hrs, cell viability was assessed using the Trypan Blue exclusion method (a) and cell damage was determined by measuring LDH release from cells using the LDH Assay (b). 10 μM 3Me-H TRH alone did not exert a toxic effect on cell viability (a) and significantly (p<0.05) inhibited cell damage (b) relative to untreated control (**). 10, 1 and 0.1 μM 3Me-H TRH, co-treated with 500 μM Glu, significantly (p<0.05) protected neurons from cell death relative to the negative control (a) as indicated by an asterisk (*). 10 and 1 μM 3Me-H TRH significantly (p<0.05) protected neurons from cell damage relative to the negative control (b) as indicated by an asterisk (*). Linear regression analysis revealed a strong relationship between presence of 3MeH TRH and maintenance of cell viability (.y=−3.857x+53.494, R2=.9143) as shown in (a) and also revealed a strong relationship between presence of 3Me-H TRH and inhibition of cell damage (y=0.0123x+0.0709, R2=.7877) as shown (b). Data are expressed as mean +/− SEM and represent between 8 and 50 replications for each treatment