Figure 4.

Enzymatic activities of circularly permuted variants. (A) Glc phosphotransferase activities of membrane preparations. Activities of the circularly permuted variants (open bars) and the fusion proteins with PhoA (solid bars) are given in percentage of wild-type (w.t.) IICB^Glc; 100% activity corresponds to 31,000 nmol Glc-6-phosphate per mg of membrane protein per 30 min for Glc and to 15,000 nmol α-Me-Glc-6-phosphate per mg of membrane protein per 30 min for αMG. (B) PhoA activities of fusion proteins between circularly permuted variants and PhoA (solid bars) and between truncated IICB^Glc and PhoA (hatched bars). PhoA activities were measured in permeabilized cells.