Table 2. Primers of SNPs PD-1.3, PD-1.5, and PD-1.6 and restriction enzymes used for RFLP analysis.
| Marker name | SNP | rs number | Location | allele | Primers | Restriction enzyme |
|---|---|---|---|---|---|---|
| PD-1.3 |
7146G/A |
rs11568821 |
Intron 4 |
A |
5′-CCCCAGGCAGCAACCTCAAT-3′ |
PstI |
| 5′-GACCGCAGGCAGGCACATAT-3′ |
||||||
| PD-1.5 |
7785C/T |
rs2227981 |
Exon 5 |
C |
5′-GTGCCTGTGTTCTCTGTGGA-3′ |
Pvu II |
| 5′-CCAAGAGCAGTGTCCATCCT-3′ |
||||||
| PD-1.6 |
8737G/A |
rs10204525 |
3′-UTR |
A |
5′-TCAGAAGAGCTCCTGGCTGT-3′ |
Nla III |
| 5′-GGGGAACGCCTGTACCTT-3′ |