Table 2.
RBR3 is required for maize cell transformation
| Construct | Embryos, transformed/bombarded | Transformation rate, % |
|---|---|---|
| RBR3 down-regulation | ||
| Control | 38/350 | 10.9 |
| Nos::RepA | 103/375 | 27.5** |
| UBI::RBR3DSH | 13/400 | 3.2** |
| Nos::RepA + UBI::RBR3DSH | 31/375 | 8.3 |
| UBI::RBR3NASx2 | 26/425 | 6.1* |
| Nos::RepA + UBI::RBR3NASx2 | 42/425 | 9.9 |
| RBR3 overexpression | ||
| UBI::GUS, control | 27/260 | 10.4 |
| UBI::RBR3HA | 28/270 | 10.4 |
| UBI::RBR3HA-C788G | 23/283 | 8.1 |
| 35S::RBR3HA-C788G | 16/281 | 5.7* |
* and **, significance levels (*, 0.05 probability; **, 0.01 probability) relative to control in each of the 2 independent experiments (RBR3 down-regulation and RBR3 overexpression).