Time-dependent induction of Tspo promoter activity by PMA. (A) Effect of PMA (50 nM) or DMSO (control) on pGL3-805 luciferase activity in COS-7 cells. (B) Dose-dependent effect of PMA on pGL3-805 luciferase activity in MA-10, NIH-3T3, and COS-7 cells. (C) Effect of PMA (50 nM) on pGL3-2700 luciferase activity in MA-10, NIH-3T3, and COS-7 cells. In B. and C., cells were treated with PMA for 24 h. Values are derived from three independent experiments (n = 9). **p < 0.01 and ***p < 0.001 vs. control; ns, non significant.