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. Author manuscript; available in PMC: 2009 Dec 3.
Published in final edited form as: Biochemistry. 2008 Dec 2;47(48):12886–12899. doi: 10.1021/bi8012643

Figure 8.

Figure 8

PKCε mediates PMA-induced Tspo promoter activity and TSPO protein levels. (A) Effect of calphostin, G06976, or RO 32-0432 on PMA-inducible pGL3-805 activity in MA-10 cells. (B) Effect of a PKCε-specific translocation inhibitor peptide (Pep Inh) or scrambled peptide (Neg Con) on PMA-inducible pGL3-805 activity. (C) Effect of RO 32-0432 on PMA-induced MA-10 proliferation. (D) Effect of down regulation of PKCε on TSPO protein levels. Cells were treated with the siRNA pool specific for PKCε or scrambled oligonucletides as described under materials and methods. At the end of the experiment cells were analyzed for PKCε, PKCα, TSPO and GAPDH expression by immunoblot analysis. Results for A-C are derived from three independent experiments (n = 9). *p < 0.05, **p < 0.01, and ***p < 0.001 vs. control. Immunoblots (D) are representative of two independent experiments.