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. Author manuscript; available in PMC: 2009 Nov 19.
Published in final edited form as: Bioconjug Chem. 2008 Nov 19;19(11):2182–2188. doi: 10.1021/bc800270w

Figure 11. Toxicity studies.

Figure 11

Cells were treated with free 623, 623 complexed with Lipofectamine 2000 (1.5 ug/ml) (L2/623) or RPA-623 under the same conditions as used for luciferase induction experiments. After 48 h, cells were trypsinized and viable cells were counted for short term studies (A, shown as bars). Alternatively cells were replated in 6 well plates containing a mixture of 1% low gelling temperature agarose and complete DMEM medium with 10% FBS for long term toxicity studies (B, shown as circles and a line). After 14 days, surviving colonies larger than 25 cells were counted. Survival is expressed as colonies per 100 cells plated.