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. Author manuscript; available in PMC: 2009 Nov 19.
Published in final edited form as: Bioconjug Chem. 2008 Nov 19;19(11):2182–2188. doi: 10.1021/bc800270w

Figure 4. Dose response and specificity studies.

Figure 4

Cells were treated with either free 623, RPA-MM, CPA-623 (prepared by conjugating cysteine with the maleimide on PEG), RPA-623 at 25 to 200 nM, or 623 complexed with Lipofectamine 2000 (1.5 ug/ml) at 100 and 200 nM (L2/623) as described in experimental procedures. Luciferase activity was determined after 72 h from the cell lysates and expressed as relative luminescence units (RLUs) per ug of protein. Results are means and standard errors of three determinations.