Table 2.
Specific activities of β2 variants.
| Proteins | Cys Variants nmol/mg/min |
BP Variants nmol/mg/min |
DAN Variants nmol/mg/min |
|---|---|---|---|
| β2(S341) | - | 3.4 | - |
| β2(N343) | 1210 | 893.7 | - |
| β2(Q345) | - | 8.8 | - |
| β2(A347) | - | 8.4 | - |
| β2(Q349) | - | 4.7 | - |
| β2(V351) | - | 74 | - |
| β2(V353) | 300 | 28 | - |
| β2(Y356) | 11 | 4 | 6.8 |
| β2(V358) | - | 43 | - |
| β2(I361) | 1091 | 161 | 533 |
| β2(V365) | 1575 | 158 | 540 |
| β2(T367) | 2586 | 482 | 601 |
| β2(L370) | - | 209 | - |
| β2(F373) | 1168 | 1093 | - |
| β2(L375) | - | 297 | - |
For comparison, wt-β2 has an activity of 5933.0 nmol/mg/min. ‘-’ denotes not determined. The lower limit of activity detection is 0.6 nmol/min/mg.