Inhibition of P. falciparum IRBC binding to CSPG by dodecasaccharides prepared by transglycosylation reaction. The C4S 12 mers containing different 4-sulfate content, prepared by transglycosylation reaction followed by HPLC fractionation (see Figure 6 and Table 2), were analyzed for inhibition of IRBC binding to the placental CSPG. The analysis was done three times each in duplicate and the results are presented as mean + SEM. +, partially sulfated C4S with 40% 4-sulfated and 60% nonsulfated disaccharides; ○, oligosaccharide 4S-0S-0S-0S-0S-0S-PA; ●, 4S-4S-0S-0S-0S-0S-PA;Δ, 4S-4S-4S-0S-0S-0S-PA;σ, 4S-4S-4S-4S-0S-0S-PA; ■, 4S-4S-4S-0S-4S-4S-PA; ▪, 4S-4S-4S-4S-4S-4S.