Table 1.
Disaccharide composition
|
|||
---|---|---|---|
C4S fraction | Δdi-4S | Δdi-0S | Δdi-6S |
% mol proportionb | |||
I | 3 | 97 | 0 |
II | 9 | 91 | 0 |
III | 14 | 86 | 0 |
IV | 26 | 74 | 0 |
V | 35 | 65 | 0 |
VI | 52 | 48 | 0 |
VII | 75 | 24 | 1 |
VIII | 86 | 9 | 5 |
The partially 4-sulfated C4S fractions obtained by DEAE-Sepharose chromatography (see Figure 2).
calculated from peak areas in the chromatogram assuming that molar extinction coefficients of non-sulfated, 4-sulfated and 6-sulfated disaccharides are similar.