Table 3.
Compositions of C4S Δ-oligosaccharide subfractions determined by mass spectrometry
| Mass
|
||||
|---|---|---|---|---|
| Δ-Oligosaccharide subfractiona | Calculated | Found | Abundance (%) | Structure assignment |
| III-F25c | 3654.6 | 3655.4 | 16 | Δ18-mer 3Sb |
| 3734.6 | 3734.4 | 51 | Δ18-mer 4S | |
| 4114.0 | 4114.6 | 33 | Δ20-mer 4S | |
| III-F26b | 3195.2 | 3195.7 | 100 | Δ16-mer 2S |
| III-F26c | 3115.2 | 3115.4 | 10 | Δ16-mer 1S |
| 3195.2 | 3195.4 | 37 | Δ16-mer 2S | |
| 3275.2 | 3275.4 | 53 | Δ16-mer 3S | |
| III-F27b | 2815.8 | 2815.6 | 45 | Δ14-mer 2S |
| 3035.2 | 3036.0 | 12 | Δ16-mer 0S | |
| 3115.2 | 3116.8 | 16 | Δ16-mer 1S | |
| 3195.2 | 3196.2 | 27 | Δ16-mer 2S | |
| III-F27c | 2815.8 | 2815.6 | 12 | Δ14-mer 2S |
| 2895.8 | 2895.8 | 26 | Δ14-mer 3S | |
| 3115.2 | 3117.2 | 07 | Δ16-mer 1S | |
| 3195.2 | 3195.2 | 19 | Δ16-mer 2S | |
| 3275.2 | 3275.4 | 36 | Δ16-mer 3S | |
| III-F27d | 3275.2 | 3275.0 | 14 | Δ16-mer 3S |
| 3355.2 | 3355.6 | 31 | Δ16-mer 4S | |
| 3734.6 | 3734.8 | 55 | Δ18-mer 4S | |
| III-F28b | 2815.8 | 2816.0 | 49 | Δ14-mer 2S |
| 2895.8 | 2896.4 | 20 | Δ14-mer 3S | |
| 3115.2 | 3116.5 | 10 | Δ16-mer 1S | |
| 3195.2 | 3196.2 | 10 | Δ16-mer 2S | |
| 3275.2 | 3275.8 | 11 | Δ16-mer 3S | |
| III-F28c | 2815.8 | 2815.6 | 19 | Δ14-mer 2S |
| 2895.8 | 2895.8 | 81 | Δ14-mer 3S | |
| III-F28d | 2895.8 | 2895.6 | 13 | Δ14-mer 3S |
| 2975.8 | 2976.6 | 29 | Δ14-mer 4S | |
| 3195.2 | 3195.6 | 21 | Δ16-mer 2S | |
| 3355.2 | 3355.4 | 37 | Δ16-mer 4S | |
| III-F29c | 2436.4 | 2435.8 | 100 | Δ12-mer 2S |
a
Subfractions from HPLC of Δ-oligosaccharide fractions obtained are presented in Table S1, and Figures S1 and S2 of the supporting information.
b
1S, 2S, 3S, and 4S in oligosaccharide length abbreviations refer to number of 4-sulfated disaccharide moiety in oligosaccharides; the remainder is non-sulfated disaccharides.