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Representative fluorescence micrographs (10X) of EGFP Expression in COS-7 cells treated with lipoplexes formed from A) pEGFP-N1 and 10 μM reduced BFDMA, B) pEGFP-N1 and oxidized BFDMA, and C) pEGFP-N1 and oxidized BFDMA and treated with a 20-fold excess of GSH following lipoplex formation. Images were acquired at 48 h after exposure of cells to lipoplexes in serum-free medium (see text).
