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. 2009 Mar;58(3):609–619. doi: 10.2337/db08-1180

FIG. 3.

FIG. 3.

Dact1 antagonizes Wnt/β-catenin signaling in 3T3–L1 cells. A: Topflash reporter activity in 3T3–L1 cells expressing control ShRNA (CtrlSH), Dact1 ShRNA (Dact1SH), empty vector (EV), or Dact1, n = 3–5 experiments performed in triplicate. Results are expressed as fold difference relative to CtrlSH. Results are the mean ± SE of at least four independent experiments. Ccnd1 (CyclinD1) (B), Id2 (C), and Pparδ (D) mRNA levels, normalized to 18S rRNA levels, were measured at the indicated hours of differentiation in Dact1SH and CtrlSH cells differentiated with MDI and in EV and Dact1 cells differentiated with DI. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. CrtlSH or EV, n = 3 experiments performed in duplicate. RU, relative luciferase unit.