Figure 5.
The effect of UK14304 and EGF on the cellular distribution of epitope-tagged α2A ARs, and GFP–EGFR proteins. Confocal microscopic images depicting the cellular distribution of HA-tagged α2A AR (a, d, and g), and EGFR–GFP (b, e, and h) before (NS; a, b, and c) and after 30-min exposure to UK14304 (d–f) or EGF (g, h, and i) in 293 cells. In the absence of agonist, both α2A AR and GFP–EGFR staining was predominantly confined to the plasma membrane (c). After exposure to UK14304, the EGFR–GFP, but not the α2A AR redistributed to an intracellular compartment (f). A qualitatively similar pattern was observed after exposure to EGF, with redistribution of EGFR–GFP but not the α2A AR (i). Qualitatively similar results have been obtained in COS-7 cells.