Fig. 1.

Extracellular matrix metalloprotease inducer (EMMPRIN) expression in transfected FaDu cell lines. (A) Western blot analysis confirms that EMMPRIN expression was reduced in the FaDu/siE cell lines, whereas control vector transfected cells (FaDu) expressed intermediate basal levels of EMMPRIN. Equal protein loading was confirmed with β-actin. (B) To confirm that EMMPRIN functionality was suppressed as well, tumor cells were plated with and without normal dermal fibroblasts (NDFs). After 72 hours, control vector cells plated with NDFs demonstrated higher growth rates compared to silenced cells (P = 0.0009).