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. 2009 Mar 13;5(3):e1000412. doi: 10.1371/journal.pgen.1000412

Figure 4. Co-regulatory transcription factors are required for proper lacZ expression in stage 11 rpr-4S3/3′ reporter line.

Figure 4

(A to F) In rpr-4S3/3′ reporter line, β-galactosidase expression in the following genetic backgrounds is shown: (A) rpr-4S3/3′ control, (B) gcmN7-4, (C) apt03041, (D) ems9G/ems7D99, (E) Df(3L)DocA, (F) Dfdw21 mutant embryos. Red boxes in (A to F) highlight maxillary segments. (A′ to F′) Close-up of maxillary segments in respective mutants. The yellow asterisks in (A′, B′, C′, D′ and F′) mark expression of lacZ in procephalic lobes. (A″ to F″) Dfd protein expression in the respective genotypes. Note that although the morphology of the maxillary segment is changed, the expression domain and intensity of Dfd protein in the respective mutants (B″ to E″) is very similar to wild-type Dfd protein expression (A″).