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. 2009 Mar 6;5(3):e1000331. doi: 10.1371/journal.ppat.1000331

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Cells were co-transfected with HA-tagged p14 and FLAG-tagged p14 endodomain, fixed and permeabilized at 8 h post-transfection, and stained using rabbit anti-HA and mouse anti-FLAG antibodies and appropriate fluorescently tagged secondary antibodies. The HA-tagged p14 (left panel) exhibited punctate, reticular staining in the cytoplasm while the FLAG-tagged p14 endodomain (right panel) was broadly distributed throughout the cytosol and nucleus. Scale bar = 20 µm. (B) Cells were co-transfected with the indicated expression plasmids (p14, full-length p14; V, empty vector; FE, FLAG-tagged p14 endodomain), and cell lysates were fractionated into the cytosolic “C” and membrane “M” fractions before being processed for Western blotting using anti-p14 or anti-FLAG antibodies (indicated on the left).