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. 2009 Mar 6;5(3):e1000331. doi: 10.1371/journal.ppat.1000331

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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QM5 cells were transfected with the FLAG-tagged p14 endodomain, and immunostained using anti-FLAG antibody and fluorescently tagged secondary antibody (A). F-actin was visualized by staining with FITC-phalloidin (B,C). A transfected cell expressing the FLAG-tagged p14 endodomain and an adjoining cell either not transfected or expressing undetectable levels of the endodomain ((A), lower and upper cells, respectively) exhibited similar arrangements of F-actin, as detected using FITC-phalloidin (B), and the same actin organization was observed in mock-transfected cells (C). Scale bar = 10 µm.