Figure 1.

Kinetics of TLS across six different types of DNA damage in human U2OS cells. Plasmid mixtures containing the indicated gap-lesion plasmid, along with the control and the carrier plasmids, were introduced into human U2OS cells. Following incubation of 0–24 h to allow TLS, the DNA was extracted and used to transform an E. coli indicator strain. The extent of TLS was calculated as described under Materials and methods. Each of the data represents the average of four TLS experiments. The detailed data are presented in Supplementary Tables 1s–6s.