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Gel retardation experiments indicating binding of the wild-type deaminase domain to a short ssDNA comprising 10 residues. 32P-labelled 10-mer DNA (200 nM), d(ATTCCCAATT), was incubated with 0, 40 and 80 μM of the deaminase domain of the wild-type APOBEC3G (lanes 1–3). The mixtures were run on a polyacrylamide gel and then exposed.
