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. 2009 Jan 22;28(4):417–428. doi: 10.1038/emboj.2008.302

Figure 8.

Figure 8

Lowering of the pHmito in permeabilised cells strongly reduces mitochondrial ATP synthesis. INS-1E cells were permeabilised with α-hemolysin toxin and analysed for mitochondrial matrix pH changes (A, B), mitochondrial membrane potential polarisation (C, D) and ATP generation (E, F). Mitochondria of the permeabilised cells were stimulated with either 1 mM succinate (A, B, C, E) or 10 mM DL-α-glycerolphosphate (D, F). (A) Nigericin caused acidification of the mitochondrial matrix at both 100 and 500 nM. (B) Quantification of the mitochondrial pH measurements (N=4–7). The same concentrations (C, D and data not shown) resulted in hyperpolarisation of the inner mitochondrial membrane (N=6–8). Nigericin strongly reduced ATP generation from both 1 mM succinate (E) and 10 mM DL-α-glycerolphosphate (F) oxidation (N=3–8). Values are presented as mean±s.e.m.