Table 2.
31P-NMR-measured ATP synthesis rate parameters in INS-1 cells
| Cellular compartment | kATP (min−1) | ATP synthesis rate (mM/min in bioreactor volume) | ||
|---|---|---|---|---|
| Glc 2.5 mM | Glc 15 mM | Glc 2.5 mM | Glc 15 mM | |
| Cytosolic (n=4) | 2.4±1.1 | 13.7±3.1* | 0.58±0.45 | 1.0±0.37 |
| Mitochondrial (n=4) | 1.9±1.2 | 4.6±1.4 | 0.93±0.61 | 2.29±0.76 |
| kATP is the unidirectional rate constant for synthesis of ATP from ADP+Pi. ATP synthesis rate is expressed in terms of mmoles ATP per litre bioreactor volume per minute. | ||||
| Values are reported as mean±s.e.m. | ||||
| *P<0.05 compared with basal measurement. | ||||