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. Author manuscript; available in PMC: 2010 Jan 15.
Published in final edited form as: J Immunol. 2009 Jan 15;182(2):1033–1040. doi: 10.4049/jimmunol.182.2.1033

Figure 3.

Figure 3

ILT4 D1–D2 are the main binding site for CD1d. (a) Binding of ILT4 mutants by CD1d. HEK293T cells transfected with GFP fusion constructs encoding ILT4 mutants were stained with PE-conjugated CD1d or HLA-A*0201 tetramers. Cartoons illustrate the structure of the constructs. Open circles represent ILT4 domains, and filled circles represent ILT2 domains. (b) Blocking of tetramer binding to ILT4-transfectants. CD1d (left panel) or HLA-A*0201 (right panel) tetramers were used to stain HEK293T-ILT4 cells in the presence of different concentrations of blocking proteins. Results from one out of three repeated experiments are shown.