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. 2008 Dec 12;18(5):956–965. doi: 10.1093/hmg/ddn423

Figure 5.

Figure 5.

Tau knockdown decreases autophagic induction and flux in NPC1-deficient cells. (A) Relative MAPT mRNA levels (mean±SEM) 72 h post-treatment with non-targeted (black bars) or MAPT siRNAs (white bars), as determined by qPCR. Targeted siRNAs significantly reduced endogenous MAPT mRNA expression in control and NPC1-deficient primary human fibroblasts by unpaired Student's t-test. (B) Human fibroblast lysates from control (lanes 1 and 2) and NPC1-deficient (lanes 3 and 4) cells were analyzed by western blot for the expression of LC3 (top) and GAPDH (bottom) 72 h post-treatment with non-targeted (lanes 1 and 3) or MAPT siRNAs (lanes 2 and 4). (C) Degradation of long-lived proteins in control (left panel) and NPC1-deficient human fibroblasts (right panel), following transfection with non-targeted (black bars) or MAPT siRNAs (white bars). Data (mean±SEM) are reported relative to non-targeted siRNA-transfected cells at 72 h. Relative proteolysis is significantly decreased in NPC1-deficient cells (P = 0.016 by unpaired Student's t-test), but not in controls (P > 0.05).