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. 2000 Jan 31;97(4):1495–1500. doi: 10.1073/pnas.040552697

Figure 1.

Figure 1

Retrovirus-mediated erbB-2 gene targeting. A431 cells were infected with E2C-KRAB- (A) or E2C-VP64- (B) encoding retrovirus. Three days later, intact cells were stained with the ErbB-1-specific mAb EGFR1, the ErbB-2-specific mAb FSP77, or the ErbB-3-specific mAb SGP1 in combination with phycoerythrin-labeled secondary antibody, and analyzed by flow cytometry. Dotted lines, control staining (primary antibody omitted) of mock-infected cells; dashed lines, specific staining of mock-infected cells; solid lines, specific staining of, respectively, E2C-KRAB- or E2C-VP64-infected cells.